Fuente: WIPO "tobacco agriculture" The invention discloses a method for simple and easy separation of tobacco bacterial wilt. The method is characterized by comprising the following steps of 1, collection of disease samples; 2, separation of pathogenic bacteria; 3, culture of the pathogenic bacteria; 4, preparation of a bacterial suspension; 5, PCR amplification and identification. The method has the advantages that disease leavesin the field are collected, veinal vascular bundles at the petioles are cut, and the characteristic that the content of ralstonia solanacearum in the veinal vascular bundles is far higher than other microorganisms is utilized to separate and purify the tobacco bacterial wilt; the selectivity of target pathogenic bacteria is strong, the purification speed is high, the efficiency is high, the separation and purification rate reaches 84% or above, and it can be ensured that the target pathogenic bacteria are the pathogenic bacteria of the tobacco bacterial wilt; by adopting the method, the targetpathogenic bacteria can be rapidly and accurately obtained, and a foundation is laid for the subsequent study; general scientific and technical personnel also grasp the method very easily.