Fuente: WIPO "tobacco agriculture" The invention relates to a method for screening tobacco plants resistant to tomato spotted wilt virus, belonging to the field of plant protection technology. The method includes five steps: nicotianabenthamiana seedling raising, preparation of resistance identification for tobacco seedlings, preparation of inoculation buffer, inoculation of tobacco seedlings and identification of disease resistance. The principle of the invention is that before the tobacco disease resistance identification is carried out, the field tomato spotted wilt virus source is activated on the nicotianabenthamiana, and the diseased tissue of the tobacco is collected as a poison source to carry out disease resistance identification.