Fuente: WIPO "tobacco agriculture" The invention discloses a method for phytophthora nicotianae inoculation of tobacco. The method for the phytophthora nicotianae inoculation of tobacco includes steps that (1) preparing for tobacco seed plantlets; (2) preparing phytophthora nicotianae zoospore suspension; (3) irrigating the roots to inoculate; (4) counting the morbidity situation, and evaluating the disease resistant result. The zoospore inoculated to the roots of the seed plantlets can be quantified rigidly, the resistant difference between the same variety due to excessive inoculation amount is avoided, and the resistance identification is more precise and effective; in the actual operation, the inoculation can be performed in quantities, the space usage is small, the labor cost is saved, and the experiment period is shortened. The method for the phytophthora nicotianae inoculation of tobacco is suitable for popularization.