Fecha de publicación: 23/01/2013 Fuente: WIPO "tobacco agriculture" The invention discloses a method for measuring the pollen density by an improved density gradient centrifugation method. The method comprises the following steps of: dissolving cane sugar into water, preparing gradient solutions with different concentrations by mass percentage, mixing pollen to be measured into 10 percent crane sugar solution, and injecting the solution into the bottom of a centrifugation pipe; and then preparing a 20-70% crane sugar density gradient centrifugation solution by a layering method, centrifugating at the speed of 4,000 to 10,000rpm for 5 to 15 min, after the pollen reaches settlement balance, keeping the pollen still, taking crane sugar solution with concentration corresponding to the layer wherein the pollen is suspended, drawing an absorbance standard curve of the pollen to be measured in the crane sugar solution with the corresponding concentration, measuring an absorbance value of the crane sugar solution at the layer an which the pollen is suspended by taking the curve as a reference through a spectrophotometer under a wavelength of 290nm, and calculating the pollen density according to the formula shown in the specification. The method is suitable for measurement of various pollen densities; a result is accurate; the repetitiveness is high; the method is easy to operate and is feasible; and the number of demanded samples is small.