Fecha de publicación: 26/06/2013 Fuente: WIPO "tobacco agriculture" The invention discloses a method for magnetically and rotatably applying bacteria on a culture medium panel. The method comprises the steps of: heating, sterilizing, drying and cooling a magnetic stirring rod to the room temperature for standby application, wherein the length of the magnetic stirring rod is less than the internal diameter of the culture medium panel; arranging the magnetic stirring rod on a central axis of the culture medium panel, dripping a bacterium suspension liquid onto a culture medium through aiming at a central point position of the magnetic stirring rod, and then, covering a petri dish cover; putting the culture medium panel on a magnetic stirring apparatus, driving the magnetic stirring rod to rotate on the surface of the culture medium panel through the magnetic stirring apparatus, and uniformly applying the bacterium suspension liquid on the surface of the culture medium panel; and after the applying is finished, taking the magnetic stirring rod out, and sterilizing and cleaning the magnetic stirring rod for being repeated use. According to the method, the magnetic stirring rod serves as an application tool, and the bacterium suspension liquid is uniformly applied on the surface of the culture medium panel through utilizing the horizontal rotation of the magnetic stirring rod. The non-contact drive of the magnetic stirring rod is ingeniously utilized to apply bacteria in a closed state, so that the bacteria application efficiency is increased, and the operation errors of operators can be reduced.