Fuente: WIPO "tobacco agriculture" The invention discloses a method for inducing phytophthora parasitica var. nicotianae to generate zoosporangia and release spores. The method comprises steps of: culturing phytophthora parasitica var. nicotianae mycelia on an oat medium prepared in a narrow mouth conical flask; preparing an induction liquid for generation of zoosporangia by selecting roots of tobacco seedlings, with 6-7 pieces of true leaves completely expanding, of a black shank infected variety; inducing the mycelia with the induction liquid to generate zoosporangia and to release spores. According to the invention, a characteristic that a black shank infected tobacco variety is more easily to generate affinity reaction with phytophthora parasitica var. nicotianae is employed, and a filtrate of seedling roots of the black shank infected tobacco variety is used as the induction liquid, so as to induce the cultured mycelia to generate zoosporangia. Proved by tests, the invention has a short induction time, high zoospore release rate, high obtained zoospore content; and scraping mycelia for culture is not needed. Therefore, the invention is of less pollution and easy to operate.