Fecha de publicación: 12/02/2014 Fuente: WIPO "tobacco agriculture" The invention discloses a method for identifying the disease resistance of tobacco leaves with brown spot disease, which belongs to the technical field of tobacco disease resistance identification. The method comprises the following steps: sterilizing and crushing healthy tobacco leaves in a mature period, and preparing a 4% to 6% solution through sterile water; inoculating hypha points of spore production of alternaria alternate to a culture medium flat plate, performing culture in an incubator at the temperature of 24 to 26 DEG C for 10 to 20 days, eluting spores through a tobacco leaf solution after spore production, and performing static culture for 7 to 9 hours to promote germination so as to obtain a bacterium suspension; when the tobacco leaves, which need to be identified, go into the mature period, sampling, and performing sterilization, airing and moisturizing treatment; adding 10 to 16 drips of 10 to 15 micro-milliliter of the bacterium suspension to each tobacco leaf, placing the tobacco leaves into a moisturizing frame or a manual climatic box, and performing illumination culture for 3 to 5 days, wherein the relative humidity of the moisturizing frame or the manual climatic box is equal to or greater than 90 percent, and the temperature of the moisturizing frame or the manual climatic box is 24 to 26 DEG C; after yellow infection spots are formed on the tobacco leaves, measuring the diameter of the spots, and determining the disease resistance. The method is high in success rate, high in reproducibility, quick and accurate in detection, and high in popularization and application value; convenience in checking and monitoring is realized; field pollution caused by artificial inoculation can be avoided.