Fuente: WIPO "tobacco agriculture" The invention discloses a method for efficiently stopping and concentrating zoospores of plant pathogenic oomycetes. The method for efficiently suspending and concentrating zoospores of plant pathogenic oomycetes comprises the steps of preparing zoospore suspension, stopping zoospores and concentrating resting spores, and specifically comprises the following steps of inoculating and culturing theplant pathogenic oomycetes to prepare a plant pathogenic oomycete zoospore suspension; stopping zoospores: placing the plant pathogenic oomycete zoospore suspension into a centrifugal tube, and forming the resting spores under the condition of instantaneous overweight and weightlessness alternative treatment; and carrying out low-temperature centrifugal concentration on the resting spores to obtain a target resting spore suspension. The method adopts an instantaneous overweight and weightlessness alternative treatment method to rapidly stop the zoospores, fundamentally overcomes the problem oflow stopping efficiency in the prior art, and greatly improves the infection activity of the resting zoospores. The preparation method provided by the invention has the advantages of good stability and high efficiency, can realize large-scale preparation, and meets the requirements of large-scale experimental inoculants, test bodies and the like.