Fecha de publicación: 04/07/2023 Fuente: WIPO "tobacco agriculture" The invention relates to a method for determining TSNAs in tobacco leaves based on one-step purification and liquid chromatography-tandem mass spectrometry, which comprises the following steps of: removing stems of baked or air-dried tobacco leaves, drying, crushing, sieving, weighing into a centrifugal tube, adding an internal standard mixed solution and water, and carrying out vortex extraction at room temperature; transferring the filtered extracting solution to another centrifugal tube, adding dichloromethane at the same time, whirling, centrifuging, transferring the lower-layer dichloromethane clear solution to another centrifugal tube, transferring the centrifugal tube to a water bath, and standing to volatilize dichloromethane; adding water to redissolve the extract and transferring the solution to a small sample injection bottle; and then carrying out chromatography-tandem mass spectrometry analysis. The invention develops a simple method for analyzing the TSNAs in the tobacco leaves, the TSNAs in the tobacco leaves are extracted by water, and the water extract is purified by a one-step liquid-liquid extraction method. According to the method, a better purification effect and better analysis sensitivity are obtained. The method is simple to operate, good in purification effect and wide in application range.