Method for detecting meptyldinocap residue amount in tobaccos

Fuente: WIPO "tobacco agriculture"
The invention discloses a method for detecting meptyldinocap residue amount in tobaccos. The meptyldinocap residue amount in the tobaccos is detected by detecting a corresponding hydrolysis product (2,4-dinitro-6-(1-methylheptyl) phenol, 2,4-DNOP) of meptyldinocap (2,4-dinitro-6-(1-methylheptyl) phenyl crotonate, 2,4-DNOPC). Tobacco samples are extracted by an improved method, sample introduction analysis is performed after heating ultrasonic hydrolysis under an alkaline condition, detection is performed by an ultra-high performance liquid chromatography-tandem mass spectrum negative ion multi-reaction monitoring mode, quantification is performed by an external standard method, results show that the linear relationship of the meptyldinocap is good within the range of 0.001-0.5mg/L, a correlation coefficient (r2) is larger than 0.999, detection limit is 3 micrograms/kg, the recovery rate of the 2,4-DNOPC is 92.37%-100.52%, and relative standard deviation (RSD) is 2.43%-3.30%. According to the method, sample pretreatment is simple and rapid, analysis time is short, sensitivity, accuracy and precision meet pesticide residue detection requirements, and the method is applicable to detection of the meptyldinocap residue amount in the tobaccos.