Fuente: WIPO "tobacco agriculture" The invention discloses an investigation method of resistance to tobacco black shank. The investigation method disclosed by the invention is not influenced by the environment, does not need manual intervention, and can be carried out in all tobacco planting areas. If morbidity of a field is not serious, judgement of the resistance level of a tested variety can also be prepared, and limitation of an existing investigation method is greatly eliminated. The investigation method is not limited to the natural environment of fields in the tobacco planting areas, and resistance to the black shank, especially No.0 and No.1, by a tobacco variety to be evaluated in the field of a same tobacco planting area can be investigated, and a scientific basis is provided for making a feasible prediction and forecast scheme and comprehensive prevention and control measures for the tobacco black shank. In addition, through comparison, a tobacco variety with excellent resistance to the black shank is selected, and a scientific guarantee is provided for research of the tobacco black shank.