Fecha de publicación: 07/08/2018 Fuente: WIPO "tobacco agriculture" The invention discloses a gene NtTPKa capable of increasing tobacco leaf potassium content and a cloning method and application of the gene NtTPKa. The nucleotide sequence of the gene NtTPKa is as shown in SEQ ID: No. 1. The cloning method specifically includes the steps of A, determining NtTPKa gene sequence; B, extracting tobacco RNA, and performing reverse transcription to obtain first chain cDNA; C, designing and synthesizing specific primers according to the NtTPKa gene sequence, and using the cDNA as the template to perform PCR amplification; D, recycling and purifying a PCR product, andconnecting the PCR product with a pTOPO vector. The cloning method has the advantages that the RNAi expression vector containing the NtTPKa gene is built, the RNAi vector is used to transform tobaccothrough an agrobacterium-mediated method to prepare a transgenic plant; the potassium content of the obtained transgenic plant is more than 20% higher than that of a control group; the result indicates that the gene NtTPKa is promising in application prospect in the cultivation of high-potassium-content tobacco.