Fuente: WIPO "tobacco agriculture" The invention relates to a detection method of fusarium proliferatum and application of the fusarium proliferatum. The detection method comprises a specific primer group: Fe-EF-spec1F/Fe-EF-spec1R. The invention has the following beneficial effects: 1, no specific primer for PCR detection of pathogenic bacteria layer fusarium of tobacco fusarium root rot exists in the prior art, and the invention realizes zero breakthrough; 2, the primer researched and screened by the invention has extremely high accuracy and specificity; 3, by comprehensively using the method disclosed by the invention, the maximum sensitivity can reach 0.1 ng/mu L; 4, the minimum time for detecting the suspected tobacco plants infected by the fusarium oxysporum is 24 hours; and 5, pathogenic bacteria can be detected in an early stage after fusarium grows out of an inoculation layer, the purpose of early detection of the tobacco fusarium root rot is achieved, and the method has very important significance and great economic significance on early detection, prevention and control of the tobacco fusarium root rot.