Fuente: WIPO "tobacco agriculture" The invention discloses an enterobacter nicotianae strain 65B7 as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides an Enterobacter tabaci strain 65B7, and the preservation number of the Enterobacter tabaci strain 65B7 is CCTCC (China Center For Type Culture Collection) No: M2019920. The invention also provides an application of the Enterobacter nicotianae strain 65B7 in the aspect of preventing and treating bacterial wilt, and also provides an application of the Enterobacter nicotianae strain 65B7 in the aspect of preventing and treating bacterial wilt. The invention also provides a bacterial agent based on the bacterial strain 65B7 of the Enterobacter tabaci and a method for preventing and treating the bacterial wilt of the Enterobacter tabaci and the bacterial wilt of the Enterobacter tabaci. Experiments prove that the strain 65B7 of Enterobacter tabaci can effectively maintain the disease index of tobacco plants suffering from bacterial wilt at a relatively low level.