Fuente: WIPO "tobacco agriculture" The invention discloses a dimethomorph colloidal gold assay kit and an application thereof. The dimethomorph colloidal gold assay kit comprises a kit body, a test strip, sample extraction liquid and sample reconstitution liquid, wherein the test strip comprises a sample absorption pad, a conjugate release pad, a reaction film, an absorbent pad and a PVC bottom plate; the conjugate release pad is coated with a dimethomorph specific antibody-colloidal gold marker; and the reaction film is coated with a detection line formed by dimethomorph hapten-OVA conjugate and a quality control line formed by a coating anti-antibody. The dimethomorph colloidal gold assay kit can achieve semi-quantitative detection of dimethomorph in tobacco, has the advantage of being fast, accurate and convenient to detect, can detect large batches of samples at the same time and can meet the requirements of the market for fast detecting a dimethomorph residue in the tobacco.