Fuente: WIPO "tobacco agriculture" The invention discloses a CYP82E10 gene missense mutant M594 capable of reducing nicotine conversion rate as well as application thereof. Relative to a CYP82E10 gene as shown in the nucleotide sequence SEQ ID NO.1, C at the 342 site in the CYP82E10 gene sequence which the CYP82E10 gene missense mutant M594 capable of reducing nicotine conversion rate contains is replaced by T, point mutation occurs, and the nucleotide sequence of the CYP82E10 gene missense mutant M594 capable of reducing nicotine conversion rate is as shown in SEQ ID NO.2. The CYP82E10 gene missense mutant M594 capable of reducing nicotine conversion rate is applied to a tobacco plant with low nicotine conversion rate. In a cloud and mist 87 material which is obtained by the CYP82E10 gene missense mutant M594 capable of reducing nicotine conversion rate, the nicotine conversion rate of the leaf is reduced by about 55 percent compared with that of the control, and the conversion rate of the nicotine is obviously reduced.