Fuente: WIPO "tobacco agriculture" The invention discloses a cultivation method for relay intercropping of flue-cured tobacco and crotalaria juncea to control tobacco root-knot nematode disease. The cultivation method comprises the steps as follows: crotalaria juncea seed treatment, tobacco field preparation, furrowing and sowing, film breaking, seedling pulling and thinning, tobacco seedling transplanting and crotalaria juncea stubble cutting. The crotalaria juncea seed treatment can effectively increase the seed germination rate; tobacco field preparation and sowing can effectively increase the seed emergence rate; film breaking, seedling pulling and thinning can effectively promote growth and development of the crotalaria juncea, keep reasonable group structures of the crotalaria juncea and improve root system biomass and root system exudates of the crotalaria juncea; pond application of paecilomyces lilacinus during tobacco seedling transplanting can enhance the synergistic control effect on the tobacco root-knot nematode disease; crotalaria juncea stubble cutting can reduce effects of upper shielding of crotalaria juncea field on growth and development of flue-cured tobacco, promote growth of root systems of the crotalaria juncea and enhance the field control effect. To sum up, the method has significant effects of inhibiting growth and propagation of root-knot nematode in soil of tobacco field and improving the yield and quality of tobacco in tobacco areas where the tobacco root-knot nematode disease frequently occurs.