Fuente: WIPO "tobacco agriculture" The invention discloses co-dominant SSR markers closely linked to tobacco TMV resistant gene N and application of the co-dominant SSR markers. Serial numbers of the co-dominant SSR markers are TM508-007 and TM508-118, and nucleotide sequences of amplified products of the co-dominant SSR markers are shown as SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 respectively. The application refers to application of the co-dominant SSR markers in detecting whether tobacco genome DNA contains the tobacco TMV resistance gene N or not. The co-dominant SSR markers have the advantages of stability, reliability, simplicity, convenience, quickness and low cost, thereby being applicable to N gene molecular marker assisted selection in tobacco TMV disease resistant breeding.