Fecha de publicación: 26/05/2020 Fuente: WIPO "tobacco agriculture" The invention discloses a co-dominant SSR marker closely linked with a tobacco multi-leaf gene mLN and an application of the co-dominant SSR marker. The numbers of the co-dominant SSR markers closelylinked with the tobacco multi-leaf gene mLN are TM-mln6 and TM-mln8, and the nucleotide sequences of PCR amplification products are respectively shown as SEQ ID No. 1 and SEQ ID No. 2, and SEQ ID No.3 and SEQ ID No. 4. The application is the application of the co-dominant SSR marker closely linked with the tobacco multi-leaf gene mLN in detecting whether the tobacco multi-leaf gene mLN exists intobacco genome DNA or not. The co-dominant SSR marker disclosed by the invention has the characteristics of stability, reliability, simplicity, convenience, quickness and low cost, so that the molecular marker can be used as an auxiliary selection application of a multi-leaf gene mLN molecular marker in tobacco yield breeding.