Fuente: WIPO "tobacco agriculture" The invention provides a co-dominant SSR (Simple Sequence Repeat) marker closely linked with a tar release amount gene qTAR in main stream smoke of tobacco and an application method. The serial numbers of the codominant SSR marker closely linked with the tar release amount gene qTAR in the main stream smoke of the tobacco are TMy81049 and TMy85286, and the nucleotide sequences of PCR (Polymerase Chain Reaction) amplification products of the codominant SSR marker closely linked with the tar release amount gene qTAR in the main stream smoke of the tobacco are respectively as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The application is the application of the codominant SSR marker closely linked with the tar release amount gene qTAR in detecting whether the tar release amount gene qTAR exists in tobacco genome DNA (Deoxyribose Nucleic Acid) or not. Compared with a method in the prior art, the codominant SSR marker has the characteristics of accuracy, high efficiency, stability, convenience, low cost and no detection opportunity limitation, so that the molecular marker can be applied to auxiliary selection of the molecular marker of the tar release amount gene qTAR in low-harm tobacco breeding.