Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco arsenic transportation gene NtNIP7-1, and a cloning method and an application of the gene NtNIP7-1. A nucleotide sequence of the tobacco arsenic transportation gene NtNIP7-1 is shown as SEQ ID (sequence identifier): No. (number) 1, and an encoded amino acid sequence is shown as SEQ ID: No. 2. The invention further discloses the cloning method of the tobacco arsenic transportation gene NtNIP7-1. The cloning method particularly comprises the steps of: extracting tobacco RNA (ribonucleic acid) for reverse transcription to form first chain cDNA (complementary deoxyribonucleic acid), taking first chain cDNA obtained by the reverse transcription as a template, designing and synthesizing a specific primer according to an NtNIP7-1 gene sequence, performing PCR (polymerase chain reaction) amplification, recovering and purifying a PCR amplification product, and performing sequencing. An arsenic content of a tobacco leaf can be reduced obviously by inhibiting expression of the tobacco endogenous gene NtNIP7-1 in a tobacco plant, and the gene NtNIP7-1 has wide application prospects in the field of low arsenic content seed breeding of plants and a great economic benefit potential.