Fuente: WIPO "tobacco agriculture" The invention discloses a cell strain capable of generating antibodies for detecting Pseudomonas amygdali and diseases caused by the Pseudomonas amygdali and application of the cell strain. The preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024169 and/or CCTCC NO: C2024170. The monoclonal antibody generated by the monoclonal antibody hybridoma cell strain can be prepared into handheld colloidal gold rapid test paper, and the pseudomonas amygdali in a sample can be rapidly and sensitively detected on site.