Fecha de publicación: 27/11/2013 Fuente: WIPO "tobacco agriculture" The invention discloses a biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and an anti-biological inoculant of the biocontrol strain YKY01, belonging to the technical field of bio-pesticides. The preservation number of the strain YKY01 is CGMCC (China general Microbiological Culture Collection) NO.7723. The anti-biological inoculant is obtained by the following steps of transplanting the strain YKY01 to a culture dish for activating, and culturing for 72 hours at 33 DEG C; transferring the activated lawn into 300ml of NA culture solution which is contained in a 500ml triangular flask, culturing for 72 hours at the rotation speed of 175 revolutions/min under the temperature of 33 DEG C to obtain primary fermentation liquor; inoculating the primary fermentation liquor into the NA culture solution in a seed tank according to an inoculation amount proportion of 1:150, thereby obtaining strain liquor for secondary fermentation after fermenting for 48 hours with the temperature of the seed tank of 32 DEG C-35 DEG C; and inoculating the strain liquor for the secondary fermentation to a fermentation tank for fermentation according to the inoculation amount proportion of 1:15, fermenting for 120 hours at the temperature of 32 DEG C-35 DEG C, and storing the strain liquor as biocontrol liquor, centrifuging for 3 minutes at 1500 rpm, and preparing with special-purpose preservative fluid to form active fungicide, so that the total concentration of viable bacteria in the fungicide is above1*1011 FU/ml. According to the biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and the anti-biological inoculant of the biocontrol strain YKY01, the cost is low, the successive cropping obstacle of the aromatic tobacco black shank can be effectively prevented, and the yield and the quality are improved.