Fuente: WIPO "tobacco agriculture" The invention relates to the technical field of plant virus detection, in particular to an antibody and test paper for detecting tobacco vein banding mosaic virus and application of the antibody and the test paper. The invention provides a monoclonal antibody for detecting tobacco vein banding mosaic virus, which is composed of a capture antibody and a detection antibody. The capture antibody is secreted by a hybridoma cell with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2023335; the detection antibody is secreted by a hybridoma cell with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2023337. Based on the monoclonal antibody, the invention also provides colloidal gold test paper for detecting the tobacco vein banding mosaic virus. The monoclonal antibody and the colloidal gold test paper can rapidly, accurately and sensitively detect whether the tobacco vein banding mosaic virus exists in a sample to be detected, and have wide application prospects in prevention and treatment of the tobacco vein banding mosaic virus.