Fuente: WIPO "tobacco agriculture" The invention relates to gene isolation and plant improvement, in particular to a spotted wilt resistant gene RTSW from tobacco and application thereof. Provided is a nucleic acid molecule comprising a nucleotide sequence selected from the following: a nucleotide sequence as shown in SEQ ID NO: 1; a nucleotide sequence as shown in SEQ ID NO: 2; a nucleotide sequence having at least 90% sequence consistency with at least one of the nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 3; and a nucleotide sequence encoding an amino acid sequence having at least 90% sequence consistency with the amino acid sequence as shown in SEQ ID NO: 3. Also provided are expression cassettes, vectors or host cells comprising the nucleic acid molecules. Also provided are methods for conferring or enhancing resistance of a plant to a virus of the genus Zhengsha, comprising introducing the nucleic acid molecule into a plant cell.