Fuente: WIPO "tobacco agriculture" The invention discloses alcaligenes aquatilis and an acquisition method and application thereof. The alcaligenes aquatilis refers to (Alcaligenes aquatilis) 05-101 which is preserved in China Center for Type Culture Collection, and a preservation number is CCTCC No.M2019665. The acquisition method includes procedures of separation and screening; firstly, strains capable of degrading nicotine are separated from tobacco plant tissues, and then, screening is performed by adoption of a culture medium with NNK as a unique carbon source and a nitrogen source. Strain application includes procedures of fermentation, inoculation and degradation; firstly, strain culture is performed at 28-30DEG C for 36-72h to obtain a fermentation bacterial agent, and in a tobacco curing or shredding process, the fermentation bacterial agent is sprayed by 3-5% of the tobacco weight, and degradation time is kept to be 4-7d (flue curing) and 45-50d (air curing). The strain is capable of realizing efficient oriented degradation of special nitrosamine in tobaccos, and high stress resistance, simplicity, convenience and easiness in acquisition, convenience in use and high popularization and application values are realized.