Fuente: PubMed "Tomato process" Fish Shellfish Immunol. 2026 Mar 28:111305. doi: 10.1016/j.fsi.2026.111305. Online ahead of print.ABSTRACTCephalopholis sonnerati, commonly known as the tomato grouper, is a highly prized edible fish with significant economic potential in aquaculture. However, its factory farming expansion is hindered by frequent diseases, notably viral nervous necrosis disease, which causes high mortality rates in fry. To advance research in immunology and virology for this species, establishing a cell line is crucial. In this study, we established the cell line derived from the tomato grouper, designated as TGB (Tomato Grouper Brain). Primary cells isolated from the brain tissue of the tomato grouper were successfully propagated through over 80 passages within 11 months. The TGB cell line grows well in L-15 medium supplemented with 8% fetal bovine serum (FBS), and exhibits a uniform fibroblast-like morphology upon confluence. Notably, the TGB cell line has a karyotype of 49 chromosomes, which differs from the typical 48 chromosomes in normal somatic cells. The cells are amenable to transfection with Cy3-labeled scrambled siRNA and the EGFP-N3 plasmid, with clear fluorescence observed at 24 hours post-transfection (hpt). Following inoculation with nervous necrosis virus (NNV), the TGB cells exhibited obvious cytopathic effects (CPE) within 12 hours post-infection (hpi), and virus particles were detected by transmission electron microscopy (TEM). Transcriptomic analysis of NNV-infected TGB cells revealed that differentially expressed genes (DEGs) were significantly enriched in pathways related to viral infection, cytokine-cytokine receptor interaction, necroptosis, and glycolysis/gluconeogenesis. The high expression levels of genes such as irf3, irf7, irf9, and cxcl10 suggest their potential roles in the cellular resistance to NNV. Therefore, the TGB cell line provides a valuable in vitro tool for biological studies, particularly those focused on the prevention and control of NNV, and offers insights into disease mechanisms and potential therapeutic strategies for the tomato grouper.PMID:41912043 | DOI:10.1016/j.fsi.2026.111305