Fuente: WIPO "tomato" Provided herein are methods, compositions, and kits for isolating extracellular vesicles (EVs). These methods and systems achieve EV isolation more efficiently, at lower cost, and with higher purity and quality of the isolated EVs, as compared to prior technologies. The disclosed methods include contacting a sample with a chitin-based stationary phase material functionalized with a fusion protein, wherein the fusion protein comprises i) a lactadherin C1C2 domain, ii) a chitin-binding domain (CBD), and iii) an intein positioned between the lactadherin C1C2 domain and the CBD.