Fecha de publicación: -- Fuente: WIPO "tomato" The invention pertains to a method for increasing the accuracy of sequencing, in particular ONT sequencing. The invention relates to a method for determining a sequence of interest in a double-stranded nucleic acid molecule, wherein the method comprises the steps of providing a sample comprising the double-stranded nucleic acid molecule, covalently closing at least one end of the double-stranded nucleic acid molecule to provide a double-stranded nucleic acid molecule having one open end and one closed end, sequencing both strands of at least part of the double-stranded nucleic acid molecule in a single sequencing reaction to generate a duplex read; and generating a consensus sequence from the duplex read to determine the sequence in the double-stranded nucleic acid molecule. Preferably, the sequencing is nanopore sequencing.