Fuente: WIPO "tomato" The present disclosure provides compositions comprising engineered integration enzymes/eLSR and methods of using the same. In certain embodiments, the engineered integration enzyme comprises mutation(s) that substantially maintain or enhance integration activity at a pair of cognate integration recognition sites, and substantially decrease off-target integration activity at a pair of off-target integration recognition sites, when compared to a corresponding large serine integrase without said one or more substitutions (cLSR). The eLSR may further comprise a stabilization domain that increases the stability of the integration enzyme as compared to integration enzymes not comprising the stabilization domain.