Fecha de publicación: -- Fuente: WIPO "tomato" Here we describe click editing, a series of genome editing approaches that combine DNA nickases (including but not limited to RNA-programmable CRISPR nickases) with DNA ligases or DNA polymerases to perform a range of different genomic edits. The edit of interest is encoded on a nucleic acid template or substrate (optionally a ‘click nucleic acid’ (clkNA) or ‘click DNA’ (clkDNA) as described herein), which can be provided in trans or preferentially recruited to the target site via a variety of nucleic acid tethering methods (optionally HUH-family endonucleases (HUHes), Telomere Binding Proteins (TBPs), or DNA- or RNA-binding proteins).