Fuente: WIPO "biotechnology" Provided are an RSV vaccine as well as a preparation method therefor and the use thereof. A Pre-F related sequence of an RSV and ferritin nanoparticles are subjected to mutation design, and a Pre-F mutant protein and a ferritin mutant are fused and expressed in eukaryotic cells, so as to obtain ferritin-PreF fusion protein nanoparticles having multiple Pre-F displayed on the surfaces in a centralized manner, and the amino acid sequence of the ferritin-PreF fusion protein nanoparticles is any one of SEQ ID No. 20-27.