Fuente: PubMed "industrial biotechnology" J Agric Food Chem. 2026 Jan 8. doi: 10.1021/acs.jafc.5c14209. Online ahead of print.ABSTRACTIn this study, the antioxidant mechanisms of yak casein-derived antioxidant peptides (YCAPs: AFK, IEQI, FPFF, LPVPQ, RELEEL) were systematically investigated using an H2O2-induced HEK-293 cell model. Treatment with YCAPs at a concentration of 200 μg/mL significantly improved cell viability and enhanced cellular antioxidant capacity, as indicated by increased activities of CAT and SOD to 0.60 ± 0.094 and 1.17 ± 0.016 U/104 cells, respectively. Concurrently, MDA and GSSG levels were reduced to 0.062 ± 0.0004 nmol/104 cells and 0.74 ± 0.26 μg/106 cells, respectively (P < 0.05). Furthermore, YCAPs upregulated the expression of Nrf2, HO-1, and NQO1, while downregulating Keap1 expression under oxidative stress conditions in vitro (P < 0.05). Molecular docking analysis revealed strong binding affinities between YCAPs and Keap1, with binding energies ranging from -7.8 to -10.1 kcal/mol, suggesting that YCAPs interfere with the protein-protein interaction between Keap1 and Nrf2. These findings indicate that YCAPs exert their antioxidant effects primarily through activation of the Keap1-Nrf2/ARE signaling pathway.PMID:41505276 | DOI:10.1021/acs.jafc.5c14209