Fuente: PubMed "industrial biotechnology" Theriogenology. 2026 Jun 15;264:118038. doi: 10.1016/j.theriogenology.2026.118038. Online ahead of print.ABSTRACTBACKGROUND: Post-ovulatory aging (POA) of oocytes impairs oocyte quality, fertilization potential, and early embryonic development, with lipid metabolism playing a central role. This study explores whether targeting lipid metabolic reprogramming can mitigate POA in porcine oocytes.METHODS: An in vitro POA of porcine oocytes model was established. Targeted lipidomics, organelle function assays, and molecular analyses were performed to assess lipid metabolism and oocyte quality. Data were analyzed using standard statistical methods.RESULTS: POA oocytes exhibited significant reductions in phosphatidylinositol (PI) and its phosphorylated derivatives (PIP2/PIP3), leading to lipid droplet accumulation, disrupted mitochondria-lipid droplet interactions, impaired fatty acid oxidation, and cellular dysfunction. Supplementation with 30 mM PI partially restored lipid metabolism, improved organelle function, enhanced antioxidant defenses, and enhanced mitochondrial-lipid droplet interactions. Consequently, sperm binding, fertilization rates, and blastocyst formation were significantly increased, while DNA damage and apoptosis were reduced.CONCLUSIONS: PI supplementation effectively restores oocyte quality, fertilization potential, and embryonic developmental competence in POA porcine oocytes. These findings suggest that targeting PI-mediated may represent a promising strategy to improve reproductive outcomes in livestock and assisted reproduction programs.PMID:42323922 | DOI:10.1016/j.theriogenology.2026.118038