Fecha de publicación: -- Fuente: PubMed "industrial biotechnology" Anal Chim Acta. 2026 Dec 15;1427:346408. doi: 10.1016/j.aca.2026.346408. Epub 2026 Oct 18.ABSTRACTAeromonas veronii is an important bacterial pathogen associated with aquatic organisms and food safety. Conventional methods for bacterial detection may require labeling, amplification, or relatively complex procedures. Fiber-optic localized surface plasmon resonance (LSPR) sensing provides a label-free optical approach for bacterial detection, while functional nanomaterial interfaces can improve the interaction between the sensing surface and target bacteria. A tapered multimode fiber-nanomaterial-doped fiber-multimode fiber (MMF-NDF-MMF) sensing platform was fabricated and functionalized with AuNPs, PdNCs, and Prussian blue cubes (PBCs), followed by immobilization of an A. veronii-specific DNA aptamer. Comparative experiments using AuNP-only, AuNP/PBC, AuNP/PdNC, and AuNP/PdNC/PBC interfaces showed that the ternary configuration exhibited the highest sensitivity among the tested configurations, with a sensitivity of 0.996 nm (log CFU/mL) and a calculated LOD of 1.7 CFU/mL in PBS over the tested concentration range of 1 × 101-1 × 107 CFU/mL. The sensor exhibited good reproducibility, reusability, and selectivity, with its response systematically evaluated under different pH and temperature conditions to establish suitable operating parameters. Tests on real food samples confirmed the practical applicability of the platform. This strategy offers label-free, and sensitive detection of waterborne pathogens and represents a promising tool for food safety monitoring, aquaculture disease surveillance, and environmental health assessment.PMID:43003565 | DOI:10.1016/j.aca.2026.346408