Fuente: PubMed "industrial biotechnology" Carbohydr Polym. 2026 Feb 1;373:124650. doi: 10.1016/j.carbpol.2025.124650. Epub 2025 Nov 3.ABSTRACTThis study utilized negatively charged carboxymethyl cellulose (CMC) polysaccharide to improve the gelling properties of myofibrillar protein (MP) derived from large yellow croaker, aiming to develop a high-performance composite gel for the intestinal-targeted delivery of puerarin (PUR). The findings indicated that incorporating 1 % CMC led to a reduction in MP particle size due to electrostatic repulsion, increased the absolute value of the zeta potential, and facilitated the development of hydrophobic interactions and hydrogen bonds. This resulted in a gel network that is denser, smoother, and more uniform. At this concentration, gel strength and water holding capacity (WHC) achieved optimal levels, while cooking loss was minimized. The incorporation of CMC resulted in a reduction of MP gel swelling and protein loss in simulated gastric fluid (SGF), while simultaneously enhancing swelling in simulated intestinal fluid (SIF). The 1 % CMC-MP composite gel containing 1 % PUR demonstrated a reduced release rate in SGF and an improved release rate in SIF, while preserving outstanding antioxidant activity. This research presents an innovative approach for the high-value utilization of large yellow croaker MP and the advancement of targeted delivery systems for active substances.PMID:41320409 | DOI:10.1016/j.carbpol.2025.124650