Fuente: PubMed "industrial biotechnology" Nanotechnology. 2025 Dec 16. doi: 10.1088/1361-6528/ae2d5d. Online ahead of print.ABSTRACTThis study presents the development of a frequency-controlled drug delivery platform employing gold (Au)melittin (Mel) conjugates loaded onto titanium dioxide nanocylinders (TiO₂ NCs) decorated with magnetic (M) nanoparticles (NPs). TiO₂ NCs were synthesized via a multi-step anodization and sonication process, providing a high surface-area scaffold for drug loading, while MNPs enabled magnetically-responsive behavior. Au NPs were functionalized with Mel, a potent antimicrobial and anticancer peptide, to enhance its stability and minimize cytotoxicity. The resulting Au-Mel-loaded MNP-TiO₂ NCs exhibited controlled drug release in response to alternating magnetic fields (AMFs), with peak release occurring within 10 minutes under stimulation frequencies ranging from 10 Hz to 10,000 Hz. FTIR, TEM, EDX, and zeta potential analyses confirmed successful conjugation and integration of all components. In vitro antibacterial assays demonstrated effective inhibition of E. coli by magnetically released Au-Mel, while cytotoxicity tests indicated selective activity against HepG2 liver cancer cells with minimal impact on HEK293 cells. This nanoplatform offers a promising solution for dual antibacterial and anticancer therapy with spatiotemporal control via external magnetic fields.PMID:41401475 | DOI:10.1088/1361-6528/ae2d5d