Fuente: PubMed "microbial biotechnology" World J Microbiol Biotechnol. 2026 May 30;42(6):316. doi: 10.1007/s11274-026-04871-8.ABSTRACTThe sustainable delivery of microbial biocontrol inoculants is crucial for improving plant health and reducing dependency on chemical pesticides. This study presents an eco-benign approach to encapsulating Trichoderma harzianum spores within bacterial nanocellulose (BNC) sheets for efficient agricultural biocontrol applications. BNC, synthesized using Komagataeibacter saccharivorans NUWB1, was utilized as a protective and biodegradable carrier material to enhance the stability and viability of Trichoderma spores. The fabricated BNC-Trichoderma composite films were characterized using FESEM, EDX, FTIR, and TGA, confirming successful spore entrapment and structural stability. Soil application tests demonstrated sustained viability and activation of encapsulated Trichoderma spores. Dual-culture assays revealed effective antagonistic activity, showing 60% inhibition of Fusarium incarnatum and 42.89% inhibition of Rhizoctonia solani. Pot experiments with soybean (Glycine max) seeds showed significant improvements in plant height, root length, and number of primary branches in composite film-treated plants under pathogen pressure compared to pathogen-only treatments. Furthermore, biodegradation studies confirmed the eco-sustainability of the fabricated films, with complete degradation observed within 28 days. This study highlights the potential of BNC-based encapsulation as a promising strategy for controlled microbial delivery, enhancing biocontrol efficacy and promoting sustainable agricultural practices.PMID:42216983 | DOI:10.1007/s11274-026-04871-8