Fuente: PubMed "plant biotechnology" Physiol Mol Biol Plants. 2026 Jul;32(7):1655-1670. doi: 10.1007/s12298-026-01773-9. Epub 2026 Jun 11.ABSTRACTTitanium dioxide nanoparticles (TiO2NPs) are increasingly proposed for agricultural applications; however, their crop-specific safety profiles remain insufficiently understood. Here, we provide, to our knowledge, the first integrative evidence that TiO2NPs exert predominantly negative effects on blueberry (Vaccinium corymbosum L.), a high-value woody perennial. In an in vitro 40-day exposure (30, 60, 90, 150 mg L-1), we quantified coordinated responses across physiological, histological, and biochemical levels. Shoot elongation declined at all doses (up to 57%), with reduced biomass at higher concentrations. Histological analysis showed progressive tissue disorganization, from mild cell compaction at low doses to necrosis and vascular damage at 150 mg L-1. Chlorophyll a, chlorophyll b, and carotenoid levels declined by up to 68%, indicating impaired photosynthetic capacity. Concurrently, TiO2NPs stimulated antioxidant defenses: phenolic and flavonoid content increased up to threefold, while superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities rose by 138% and 147%, respectively. Correlation and sensitivity analyses confirmed strong antagonism between growth and defense responses. Shoot length positively correlated with pigment content (r = 0.99) but negatively with SOD (r = - 0.87) and flavonoids (r = - 0.75), suggesting a trade-off between growth and oxidative stress mitigation. SOD, APX, and carotenoids emerged as the most responsive biomarkers. Collectively, these multi-level data demonstrate that TiO2NPs inhibit development and disrupt tissue integrity in blueberry, supporting a precautionary perspective: TiO2NP-based fertilizers are not recommended for blueberry cultivation until safe exposure thresholds are validated under soil and field conditions.SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-026-01773-9.PMID:42434179 | PMC:PMC13350783 | DOI:10.1007/s12298-026-01773-9