Fuente: PubMed "plant biotechnology" Int J Syst Evol Microbiol. 2026 Mar;76(3). doi: 10.1099/ijsem.0.007100.ABSTRACTTwo novel bacterial strains designated CR2-8T and HWE2-09T were isolated from mountain soil and plant root, respectively, and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CR2-8T shared the highest sequence similarity with Sphingobium limneticum 301T (98.0%), and HWE2-09T with Sphingobium rhizovicinum CC-FH12-1T (97.8%). The genome-based comparison indicated that both CR2-8T and HWE2-09T shared the highest relatedness with Sphingobium cupriresistens CU4T, sharing 84.05% and 84.7 % orthologous average nucleotide identity and 29.3% and 30.3 % digital DNA-DNA hybridization, respectively, all of which were clearly below the suggested cutoffs for species distinction. For both strains, the major fatty acids were two summed features, one consisting of C16 : 1 ω7c and/or C16 : 1 ω6c and the other consisting of C18 : 1 ω7c and/or C18 : 1 ω6c, and also C14 : 0 2OH and C16 : 0. The major ubiquinone was Q-10, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyldimethylethanolamine for both strains. The DNA G+C content of strain CR2-8T was 63.16 %, and that of strain HWE2-09T was 63.26 %. The phenotypic, chemotaxonomic and phylogenetic analyses indicate that each of the strains CR2-8T and HWE2-09T should be classified as a new species of Sphingobium, for which the names Sphingobium wonjuense sp. nov. (type strain=CR2-8T=KCTC 8939T=JCM 36935T) and Sphingobium erigeronicola sp. nov. (type strain=HWE2-09T=KCTC 8940T=JCM 36936T) are proposed.PMID:41802012 | DOI:10.1099/ijsem.0.007100