Fuente: PubMed "nature biotechnology" 3 Biotech. 2026 Jan;16(1):46. doi: 10.1007/s13205-025-04666-y. Epub 2026 Jan 5.ABSTRACTSustainable alternatives to fish meal are increasingly required to minimize the environmental impact of aquaculture feeds. This study focuses on incorporation of microalgae Spirulina platensis and Chlorella vulgaris in rainbow trout's (Oncorhynchus mykiss) feed replacing 25 and 50% of fish meal present in standard formulation and reaching total microalgae proportion in feed, respectively 5 and 10%. It was demonstrated that trout with initial weight 45 and 65 g has significantly improved growth (weight and length) when fed with microalgae supplemented feed. The highest gains in length and weight in both groups were observed using 5% S. platensis feed, with average wight increase up to 28% compared to the control. Incorporation of microalgal biomass has significantly improved the chemical composition of trout's filet, due to increase in concentration of certain PUFA (including DHA, EPA, Omega-3, Omega-6), carotenoids, vitamins and iron. Enhanced filet pigmentation was also observed. Histological analysis showed a significant improvement in villi diameter and goblet cell count in pyloric appendages and proximal intestine. To sum up, partial fish meal substitution with microalgae has significantly improved trout growth performance and product quality and has strong potential to reduce the environmental impact associated with overfishing.SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-025-04666-y.PMID:41502485 | PMC:PMC12770158 | DOI:10.1007/s13205-025-04666-y