Fuente: PubMed "medicinal and aromatic plants" World J Microbiol Biotechnol. 2025 Nov 13;41(11):452. doi: 10.1007/s11274-025-04665-4.ABSTRACTThis study explores the extraction, optimization, and application of crude broth was used for soil/plant treatment, reserving purified EPS only for structural analysis produced by Kocuria carniphila AUMC B-453, isolated from Egyptian agricultural soil and identified via 16 S rRNA sequencing. Characterization using Fourier Transform Infrared Spectroscopy (FTIR), Proton nuclear magnetic resonance (1HNMR), and High-Performance Liquid Chromatography (HPLC). confirmed the EPS as a heteropolysaccharide composed predominantly of arabinose, ribose, glucose, mannose, and xylose. Using the Plackett-Burman design, optimal EPS yield (13 g/L) was achieved with 30 g/L glycerol, 2 g/L yeast extract, pH 7, and 48 h incubation. Among substrates tested, glycerol and yeast extract were the most efficient carbon and nitrogen sources, producing 8.6 g/L and 10 g/L EPS, respectively. SEM analysis revealed a fibrous polymer matrix bridging sand particles, FTIR indicated strong chemical interactions between EPS and soil minerals. Under low irrigation, plants in EPS-treated soil showed increased growth: 17.7 cm shoots and 23 cm roots at 250 mL/2 kg soil. EPS treated soil demonstrated enhanced water retention, aggregation, and nano-porosity. These effects are attributed to dual physical and chemical mechanisms, contributing to reduced water loss and improved soil structure. The findings validate K. carniphila EPS as a potent, sustainable soil conditioner. This work supports microbial EPS as a promising tool for improving agricultural productivity in arid and sandy environments.PMID:41231369 | DOI:10.1007/s11274-025-04665-4