Fecha de publicación: -- Fuente: PubMed "essential oil" J Ethnopharmacol. 2026 Nov 6:122591. doi: 10.1016/j.jep.2026.122591. Online ahead of print.ABSTRACTETHNOPHARMACOLOGICAL RELEVANCE: Androctonus crassicauda and Mesobuthus gibbosus are scorpions with powerful toxins with causes of envenomations leading to death but they are also potential candidates for drug development. Pharmacological investigations on venoms of Androctonus crassicauda (Acr) and Mesobuthus gibbosus (Mgb) are few. Ethnopharmacological use of Origanum species against envenomations was noted by Dioscorides.AIM OF THE STUDY: Aim of the study was to investigate actions of Acr and Mgb on prostatic and epididymal segments of isolated rat vas deferens and to investigate effects of Origanum onites essential oil (OOeo) against actions of these venoms.MATERIALS AND METHODS: Acr and Mgb were tested on phenylephrine- CaCl2- and KCl-induced contractions of prostatic and epididymal segments of isolated rat vas deferens. Effects of OOeo on actions of venoms were tested. Regular expressions were used for short linear motifs and Clustal omega for multiple sequence alignment of toxins and TRPA1.RESULTS: Both venoms inhibited phenylephrine-induced contractions but augmented KCl-induced contractions. CaCl2-induced contractions were not affected. Parallel shift of dose-response curve of Mgb was found. Molecular mimicry using GxxxG (GG4) motif that has the structure [GS]xx[FY]GYC was found indicating role of TRPA1 ion channels. This motif was evolutionary conserved from Cnidaria to mammals. Dose-dependent inhibitory actions of OOeo against Acr-induced contractions of epididymal vas deferens indicated possible roles of TRPA1 and voltage-dependent Na+-channels.CONCLUSION: Contractile actions of the venoms on vas deferens and dose dependent inhibitory actions of OOeo against these venoms supply pharmacological evidence for the ethnopharmacological use of OOeo.PMID:42994576 | DOI:10.1016/j.jep.2026.122591