Fuente: PubMed "essential OR oil extract" Nanomedicine (Lond). 2026 Jul 11:1-15. doi: 10.1080/17435889.2026.2698783. Online ahead of print.ABSTRACTThe evolution in the perspective of regulatory and industry over the last decade has led to a shift toward the adoption of new approach methodologies (NAMs), heightening the need for robust human-relevant models for clinical and basic research. As a result, the NAMs field is littered with a plethora of different approaches toward creating human-relevant testing methods. To realize the opportunity that NAMs pose, there is an essential need to have fundamental experimental strategies. This review focuses on such fundamental concepts and considerations needed to strengthen current and future techniques. Herein, a number of simple, manageable practices, which are often overlooked, are proposed, such as choosing appropriate authenticated cells, using defined media and consumables, controlling passage number, and applying suitable controls. In addition to these practical decisions, the importance of transparent reporting and dissemination, and need for all involved within research to encourage robust in vitro methods are emphasized. By elevating these 'basic' components from assumptions to explicit requirements, researchers can make impactful changes to ensure the robustness, reliability, and reproducibility of in vitro NP pulmonary toxicity studies.PMID:42434807 | DOI:10.1080/17435889.2026.2698783