Fecha de publicación: 09/12/2022 Fuente: WIPO (eseential oils OR extracts) The present invention is the result of an eco-friendly and plant-based approach. The formulated herbal product was based on traditional practices through scientific approach using the extracts from orange fruit peels, mugwort (Artemesia) and marigold which was further evaluated for mosquitocidal properties. Two dimensional structures of all the compounds present in Orange, Artemisia and Marigold were obtained from NCBI PubChem database, followed by Molecular Interaction Analysis and Molecular docking which was performed to determine the interactions between the receptor and ligands. It was inferred that Limonene was responsible for highest larvicidal activity in Orange; similarly in Marigold it was Leutin and in Artemesia it was the Artemisinin that depicted the maximum binding affinity. This means that the interaction with the receptor was stronger compared to other phytochemicals. Different combinations of these extracts were prepared in varied ratios and tested for its effectiveness against Anopheles stephensi mosquito larvae. The formulation showed good efficiency and a working span of 24-48 hours. Normal Human Dermal Fibroblast (NHDF) cells were procured from ATCC and stock cells were cultured in FGM supplemented with 10% inactivated Fetal Bovine Serum, Penicillin and Streptomycin in a humidified atmosphere of 5% CO2 at 37°C until confluent. The formulation did not show significant inhibition in NHDF cells, thus marking it dermatologically safe and non-toxic to humans. The developed formulation proves to be a safer alternative to mosquito control strategy using chemicals which when continuously used can cause development of resistance in vector species, biological magnification of toxic substances through the food chain and adverse effects on environmental quality and non-target organisms including human health.