Fuente: WIPO "essential oil or extracts" This invention relates to a method for extraction of essential oil extract from pleurospermum brunonis and uses thereof. Fungal infections are widespread and cause serious infectious diseases. The increased prevalence of antibiotics has led to development of resistance in fungi against existing antibiotics and have side effects especially hepato-toxicity. Therefore Essential oils can be explored as a resource to enhance or modify the potency of antibiotics. Essential oils are partially pure, non-toxic and free from side-effects, they can be used directly to develop improved topical anti-fungal formulations. In the present invention essential oil extracted from the medicinal plant Pleurospermum brunonis var. Benth of Apiaceae family for antifungal properties and its applications in pharmaceutical industries. Analysis of antifungal activity of essential oil extracted from leaves of Pleurospermum brunonis was done by agar well diffusion method against three fungal strains Saccharomyces cerevisiae (H1086), Candida albicans (ATCC90028) and Candida albicans (MTCC277). The Minimum Inhibitory Concentration of essential oil from Pleurospermum brunonis Benth was found to be 0.15% for C. albicans (ATCC90028), S. cerevisiae but for C. albicans (MTCC277) the value were 0.07% but whereas, for antibiotic (Fluconazole of 10mg/ml) was found to be 0.015% for S. cerevisiae and 0.62% for C. albicans (ATCC90028) but for Amphotericin B (10mg/ml) 0.31% for S. cerevisiae (H1086) and 0.62% for C. albicans (ATCC90028).