Fuente: WIPO "essential oil or extracts" The present invention discloses a method (100) for formulation for analysis of anti-glioblastoma activity of essential oil extracted from Aegle marmelos, comprises steps of, washing fresh Aegle marmelos leaves to remove dirt and debris from surface of the leaves, absorbing excess amount of moisture from the surface of the Aegle marmelos leaves, mixing a pre-defined amount of Aegle marmelos leaves in a pre-defined amount of distilled water inside a bottom flask, boiling a mixture of the Aegle marmelos leaves and distilled water at a pre-set temperature inside a Clevenger apparatus, extracting essential oils from the mixture and separating from a hydrosol, treating Ln-229 cell type with the extracted essential oil by dissolving the essential oil using Dimethylsulfoxide (Dmso) and performing a cell viability assay over the extracted essential oil to assess cellular change in Ln-229 cell type after dissolving with the extracted essential oil.