METHOD FOR CONSTRUCTING OENOCOCCUS OENI ENGINEERING BACTERIA AND USE THEREOF

Fecha de publicación: 03/06/2021
Fuente: WIPO Wine
Provided are a method for constructing Oenococcus oeni engineering bacteria and the use thereof. The construction method comprises the following steps: (1) preparing a lipase gene lipase; (2) preparing a homologous arm gene having the lipase gene; (3) preparing a plasmid pMG36e-lipase; (4) preparing a nisl fragment; (5) preparing a linear fragment containing no erythromycin sequence; (6) preparing an expression vector pMG36n-lipase; and (7) transforming the expression vector pMG36n-lipase into Oenococcus oeni competent cells to obtain the Oenococcus oeni engineering bacteria. The Oenococcus oeni engineering bacteria with the function of degrading esters obtained by means of the construction method can be applied to the production of wine, such that adverse effects on the taste and aroma of the wine caused by excessive esters in the wine-making process are avoided in order to reduce the preservation time of the wine and shorten the production cycle of the wine while achieving the same taste.