Tomato zonate spot virus RT-LAMP detection method, primer group and application of tomato zonate spot virus RT-LAMP detection method

Fecha de publicación: 11/12/2013
Fuente: Wipo tomato
The invention discloses a tomato zonate spot virus RT-LAMP detection method, a primer group and application of the tomato zonate spot virus RT-LAMP detection method. The inventor adopts the LAMP (Loop-Mediated Isothermal Amplification) technology and designs the corresponding RT-LAMP detection primer group according to the gene sequence of an L-fragment of the tomato zonate spot virus, the primer group comprises an outside primer pair F3 and B3 and an inside primer pair FIP and BIP, and accordingly the RT-LAMP detection method for detecting the tomato zonate spot virus and a kit are invented. When the detection method and the kit are used, whether tomato zonate spot virus infection exists or not can be rapidly detected only by extracting total RNA of a suspected infected plant sample like tobacco, hot pepper, tomato and weeds and conducting the LAMP, so that the basic level can conveniently, rapidly, accurately and easily identify the virus so as to take proper prevention measures and reduce the loss brought by the disease.