Fecha de publicación:
Fuente: Wipo tomato
The invention discloses a detection method of main spoilage microorganism in tomato paste and aims at no report about the detection method of main spoilage microorganism in tomato paste at home and abroad. The method is carried out as follows: by adopting the pre-breeding cultivation, the 8 to 12 percent by weight in volume of tomato paste is taken, and physiological saline of 200 to 250 mililiter is added into the tomato paste and well-mixed to form suspension; 8 to 12 percent of the culture suspension solution is respectively inoculated into the breeding culture solution of flatsour bacterium or the agar culture solution of thermophilic acid-fast bacillus and tryptic soytone broth or BCP modified culture solution, cultivated in 35 to 37 DEG C for about 24 to 48 hours, then the culture solution of being same to that of pre-breeding is isolated and cultivated; by a series of biochemical test, spore bacteria and Staphylococci is identified to mainly exist in the tomato paste and as the main spoilage microorganism. The invention takes very important role in promoting the rapid development of tomato paste industry and keeping the dominant position of the tomato paste trade in international market.